We are witnessing silent epidemic of Vitamin D deficiency in India. Any solution to problem of RO water and loss of minerals?
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?
31 July 2024 5,824 0 View
There is rapid surge of dengue cases in Southern parts of India. However there are no cases till now in the setting I work in Northern India. Besides dengue surveillance and local preventive...
14 July 2024 2,935 2 View
I have read some papers but i didn't get proper answers. In some cases, my level of theory is not exactly written. I will be very gladfull for any recommendation
10 June 2024 9,929 1 View
I am using the liquid electrolyte EC:DMMP(50:50 wt%) mixed upto 1M LiTFS. Usually, the electrolyte obtained was colorless ...but the Last two times, it gave a yellowish color. Why?
01 June 2024 8,355 1 View
I have intel iris xe graphic card. i want to run dft calculation in gaussian using a gpu. what are the necessary modifications to the input file? my input file read like...
21 May 2024 3,402 6 View
If we get a unsatisfactory water report from water supply from a locality? What preventive measures should be taken in already scarce water situation?
27 April 2024 851 0 View
I have collected data from the field in consecutive days. I have those data in excel how can i calculate the exact day of 75% seed germination using any formula.
17 April 2024 1,077 3 View
We are witnessing rising incidence of mumps . Adults are also getting complicated mumps in form of orchitis. Any reasons to explain?
07 April 2024 8,983 0 View
while trying to generate CGenFF topology for Ligand after extracting from docked file, i got error which i'm showing as a picture. What could be the possible reason and how will i rectify it for...
26 March 2024 2,491 3 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?
14 June 2024 3,254 2 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hello, I discovered that a compound that I use, which get integrated into RNA, might have an unspecific impact on mRNA stability of a gene of interest. To confirm that, I pretreated my cells for...
10 June 2024 276 2 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hello everyone, I am currently 26 years old (feeling like I am making a lot of mistakes at this young age), finishing my Master's degree, and planning to continue onto a PhD and possibly a...
30 May 2024 6,980 2 View
I am already using BL21(DE3)pLysS cells but I am still getting leaky expression in pre-expression samples before IPTG is added. LB broth is tryptone, salt and yeast. Thanks :)
28 May 2024 2,820 2 View