Western blot for Candida Albicans ?

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High Power control for 48V battery pack - High current control?

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20 January 2021 1,717 4 View

GROMACS: Cannot rename checkpoint file; maybe you are out of disk space?

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03 January 2021 3,544 2 View

How can the resistance of paving asphalt be increased to acid rain?

Modification of the rheological properties of asphalt

25 December 2020 7,390 2 View

Can someone please share the matlab code of FBMC/OQAM PAPR reduction with TR and PTS methods?

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19 December 2020 7,764 1 View

Appreciate the quality qualities of cheese ?

How to estimate the acidity of a cheese?

13 December 2020 4,890 4 View

What is the appropriate polymerase to amplify gDNA greater than 10kb?

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How to get the data of the changing the distance between two Pionts with the applied force?

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23 November 2020 352 3 View

Why I got low CI in the EBSD?

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26 August 2020 3,547 4 View

Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

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Hi Is it needed to etch a sample in order to see dislocations? SEM or TEM microscope should be used for that?

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06 August 2020 9,703 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

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Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

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How optimize the Size Exclusion Chromatography?

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Why are my bands weird in this Agarose gel?

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What do I do with DLS peaks that are from the buffer?

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What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

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26 February 2021 7,129 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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What is the best way to manage Western blots?

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23 February 2021 8,609 3 View

Karen A. Darbinyan

Hello, see info attached may be useful for you.

Good luck!

Shamsher S. Kanwar

You may collect the C. albicans cells in a test tube. Wash the cells adequately twice with 0.05 M phosphate buffer pH 6.5 and directly lyse/ homogenize the cells in SDS-PAGE buffer. Heat for 3-4 min in boiling water bath, centrifuge the cells debris and use the supernatant to load on SDS-PAGE, and thence perform WB.

Ammar Abou Kandil

Shamsher S. Kanwar what is mean "lyse/ homogenize the cells in SDS-PAGE buffer."? how i can lysis with this buffer, and what is the percentage of the SDS-page buffer?