Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit too concentrated, I am betting I might not see that weak 212 peak if they were properly diluted. So I'm wondering if this should be scored as an actual allele in those top two samples. Thanks for any suggestions.
Hi
The additional peak is very weak. In a heterozygote sample the two peaks should be almost similar intensity. It may also be a contamination, or product generated from slippage during PCR. See the profiles of confirmed heterozygous samples on your capillary system, and rule out all other possibilities before concluding
Vicky Hollenbeck Sometimes heterozygous genotypes have complicated interaction between the alleles in PCR. You can mix DNA of the samples with strong 200 bp and 212 bp and amplify it in comparison with original DNA.
- Badges
- Science topic
- Similar topics
- Computer Science
- Images