Vicky Hollenbeck

4 Questions 6 Answers 0 Followers

Questions related from Vicky Hollenbeck

Sequencing microsatellite pcr products?

I am using developed markers from a closely related subspecies (stem rust fungi) and have 24 markers that are working well in 3 host species, all amplifying in the correct patterns expected (e.g....

01 February 2022 4,975 4 View

SSR marker with 3(?) peaks in a diploid - get rid of it?

I am trying to determine the usefulness of the marker shown in the attached photo. You will see that in one sample there appears to be 3 alleles (this is a diploid, or more precisely a dikayotic...

18 August 2021 6,334 3 View

Determining linkage in microsatellite markers?

How important is it to determine linkage in a set of microsatellite markers? I am using a set of markers developed from a closely related species. The ssr markers were gleaned out of genomic...

06 May 2021 5,680 3 View

Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

28 October 2020 2,442 3 View