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I am using pichia to express protein. After using the bead beater, I found some of my protein has been denatured. I used 1 min break and 3 min cooling for 3 times. I am wondering if I miss...
15 October 2015 142 5 View
I have a question about the storage of biobeads. I am worried about the deteriorate of biobeads which may affect the proteoliposome function. I usually store them in buffer at 4 degree. I read...
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Hi, I am a postdoc working with proteoliposome. However, I have not been able to make proteoliposome with my membrane protein. Here is my general procedure. a, dry the lipid as the thin film on...
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