When comparing two very similar surveys taken over time, but with different sample size, what would be the best way to approach this problem?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a (year-long) course were given a link to...

08 December 2020 6,624 9 View

When analysing survey using Likert scale, is it okay to use Friedman's test?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a course were given a link to participate...

06 December 2020 6,060 2 View

How to use CCDC to find CIF files?

Seems like a silly question but I'm having trouble with searching for CIF files via https://www.ccdc.cam.ac.uk. I've used it before but I was on university campus so I think I was able to use more...

25 March 2020 1,271 3 View

Did anyone ever tried to extract springtails from muck soil with Berlese funnel?

We tried this last summer, but with no results, even if we were able to see them on the ground. We have already test for different heat sources (60W, 25W, 10W)...It seems that nothing is going...

01 February 2019 1,960 2 View

Why do I get variation in ELISA (sandwich) results?

Hello, we have been performing in our lab a validated ELISA test and we are getting a lot of variation in results. We have a std curve with each test and we also include a control sample and we...

02 March 2018 3,014 5 View

Can the result from Zetasizer analysis considered as the size of the nano particle?

11 May 2017 5,099 9 View

What is the process used to coat Ag with PVP? Could one just mix PVP with AgNP in distilled water and shake it?

Appreciate any input

10 January 2017 923 3 View

Is there a statistical violation in using unchosen answer selections as "0"s to allow for a rmANOVA?

Here's the study design: Participants are asked to select up to three (of 23) potential colors for an emotion. For each color they pick, they indicate on a scale 1-10 about how much they think...

25 September 2016 2,995 4 View

How to measure magnetic nano particle (iron) grain size without chemical compound?

Since SEM and TEM both have metal probe, I am wondering if there is any other way to measure magnetic nano particle (iron) grain size without chemical compound. I know that XRD could be an option...

12 July 2016 8,071 1 View

Perform classification data mining on attached dataset using Weka to build model that could be used to predict lateness?

Please help!!! I am new to Weka, and have to do a project for school regarding classification. Is there anyone familiar with Weka that would be willing to point me in the right direction? Thank...

19 April 2016 4,144 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View