Annie Newell-Fugate

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Questions related from Annie Newell-Fugate

We culture SQ and visceral preadipocytes and have a hard time getting visceral to grow and induce to adipocytes (rodent and porcine). Suggestions?

We use standard adipocyte induction cocktail for the the animal cells. The SQ changes readily from preadipocytes to adipocytes and the predadipocytes from SQ also plate down better.

08 February 2017 7,587 1 View

Does anyone have input on the best way to extract protein from white adipose tissue?

I have tried homogenization, followed by centrifugation at high speeds for 40 minutes, followed by centrifugation through a concentrator filter. I suspect my BCA assay was not accurate as I had...

02 January 2015 2,228 4 View

Has anyone had success with ceiling culture of mature adipocytes from primary tissue?

I have tried the Zhang et al 2000 protocol with flasks but have had only stromal cell looking things attach...

27 May 2013 2,412 3 View