We are looking for a spectrophotometer to measure antioxidant and phenolic content of tomato samples with. Does anyone have any recommendations as to which company and model to go with? Thank you in advance!
The best would be the company which serves your university If the low cost is your main criteria, then check Ocean Optics fiber spectrophotometers https://www.spectrecology.com/
Talk to Mike Morris, the founder of Ocean Optics, and owner of spectrecology.com. He is very knowledgeable and can help you to choose the right instrument. The price tag is several times cheaper than that of traditional instruments. You will be surprised how small are these things.
I know Mike Morris personally. He helped us to design one of our set-up and became a co-author of this publication Phys. Chem. Chem. Phys., 2014,16, 11942-11949
We use Shimazu UV-1800 and Thermo Fischer Multiskan Spectrophotometers. Either one is ok for antioxidant and phenoic studies.
Hello Brittany;
It depends on the scale of your antioxidant and phenolic experiments. If you will analyze out a large number of samples at once (e.g., many treatments and replicates), I would recommend that you buy a microplate reader spectrophotometer which can accommodate up to 99 or 340 samples per run. Most of these instruments are equipped with automatic temperature control and shakers. These are also useful to carry out kinetics measurements as for the antioxidant capability assay. Of course, these instruments are a bit more expensive that a standard cuvette spectrophotometer, but they are a must for high-throughput analyses. As Ercan Bursal, I would recommend a Multiskan Spectrophotometer from Thermo Fisher Scientific. The basic model is the Multiskan FC (microplate photometer) which can accommodate up to 99 samples, with shaking capability, This instument uses light filters and has a wavelength interval from 340 to 850 nm. It comes with the following filters: 405, 450 y 620 nm.
https://www.fishersci.es/shop/products/multiskan-fc-microplate-photometer/p-4530543#?keyword=multiskan
The more advanced Multiksan GO (microplate spectrophotometer) can accommodate up to 99 or 340 samples, has a monocromator with a wavelength interval from 200 to 1000 nm (no need for filters). It is also equipped with an incubation system, up to 50 °C.
https://www.fishersci.es/shop/products/multiskan-go-microplate-spectrophotometer/15224687?change_lang=true
For a reduced number of samples, you can work with a standard cuvette spectrophotometer. I personally have in my lab a very good spectrophotometer I which I highly recommend since it is very economical (as compared with other commercial trademarks), easy to use, and very reliable. It is the Thermo Scientific™ GENESYS™ 50 UV-Vis spectrophotometer.
https://www.fishersci.es/shop/products/genesys-40-50-vis-uv-vis-spectrophotometers/p-8430009#?keyword=spectrophotometer
Hope this helps. Regards.
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