Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View

Why values of functional dispersion index appears to be very high?

07 October 2019 9,862 3 View

What is the lowest yield of RNA that can be measured (for integrity) on a Denaturing Agarose gel?

Hi everyone, After RNA extractions and DNase treatments, I measured my samples for RNA yields in NanoDrop. On average they are 10ng/ul (ranging from 2ng/ul to 29ng/ul). Very low. 260/280 ratio...

23 January 2019 7,243 1 View

Does anyone know of a bioinformatic tool to design a new interaction pathway of genetic expression?

For example about SENP1 gene. How much do you know it? I need information about genetic mechanism of SENP1 gene, maybe anyone know about how to relate between molecular interaction pathways as are...

27 May 2014 6,861 1 View

Are there any specific cell surface markers for macrophages and DC (both from RAW 264.7)?

I need to know what are the main markers for identifying macrophages and DC from RAW 264.7 activated in vitro. We intend to observe expression of some surface markers on different phenotypes...

09 January 2014 4,791 11 View

Does anyone know a Tag antibody showing NO cross-reaction in Xenopus oocytes?

Better if it is a commercial antibody.

18 November 2012 6,748 4 View

Anyone know a good tutorial to simulate two proteins in the same box in Gromacs? or how to merge two files .gro?

I'm still learning and already have a bad time trying to find a solution to this issue.

01 January 1970 4,850 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View