How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

Are there commercially available fluorescence-tagged Influenza A virus?

30 November 2020 9,445 1 View

What tests for: 1. differences between continuous variables when grouped with different categorical ones; 2. significance of potential relationships?

Hello. I was wondering if anyone had suggestions in terms of what exploratory analysis to carry out for my dissertation with the following data. I measured N=26 participants pre and post an...

09 August 2020 5,256 5 View

SURVEY - how people changed attitude toward green spaces & urban forests during the COVID19 quarantine?

https://vub.fra1.qualtrics.com/jfe/form/SV_5bSsVfI1g1VgI7j While shaking our society and turning our way of living upside down, the COVID19 pandemic is also putting the relation between nature...

15 May 2020 2,339 8 View

Direct Input of the Occupation Matrix?

I'm implementing the Occupation Matrix Control scheme (OMC) in VASP DFT+U calculations via direcct input of the Occupation Matrix. This requires an external input file (OCCMATRIX) containing the...

17 March 2020 1,921 1 View

Glycerol topology/structure for GROMOS87/Berger forcefield?

Hello all, I need the topology /structure file of the glycerol for the GROMOS87 forcefield. Is some repository available online or do someone know how can I derive them by myself (maybe some tool...

12 March 2020 8,858 2 View

How to interpret moderating effect in PLS?

I am running a Partial Least Squadre Path Modelling in R and I have a Moderating effect (explained by one scalar variable) between my exogenous x and my endogenous y. The only variable that...

25 February 2020 8,022 4 View

Recombinant protein for antibody neutralization?

I need to understand if an antibody I'm using is specific or not. The antibody is not validated. In immunoblotting, the antibody detects a prominent band at the right molecular weight and two...

29 April 2019 4,752 4 View

How do I isolate LC nucleus for cell culture?

I need to isolate LC for primary cell culture? How should I do? What is better: use frozen tissue or fresh tissue?

21 January 2019 9,383 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View