wat is the influence of pH of mdium extraction solvent in phenolic compounds and antioxidant activity?
The extraction yield of phenolics and antioxidant activity changes with change in pH of the extraction media.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635725/
from practical prospective i faced technical difficulties with some methods which contain acidified aquas ethanolic or methanolic extraction solvents, such like this method (One normal solution of HCl (1N)/95% ethanol (v/v, 15/85) was used for extraction. The extraction procedure involved addition of 20 mL of solvent to 3.0 g of sample) . On the other hand other methods demand HCl Tris buffer 7.4. but
when i used aquas ethanolic or methanolic solvents without acidification i got realable better results.
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