What is the best buffer for washing blood cells prior to cytospin and fixation? I am not sure whether I use PBS or HBSS?
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...
27 April 2024 4,710 1 View
I am hoping that someone can help me find an antibody for GABA, GAD 65 or GAD 67 that works well in marmoset cerebral cortex Thanks
21 February 2024 7,493 2 View
Hello, I am looking for a dataset of requirements gathering scripts and user stories extracted from those requirements. Anything similar would be helpful. Thank you.
01 February 2024 4,604 0 View
Hello , I hope this message finds you well. I'm a final-year civil engineering student working on a dissertation about the sustainability of ancient Egyptian construction techniques and their...
14 November 2023 987 1 View
I used some polyclonal western blot antibody, and that datasheet size is 45-50kDa I also get the band in expected size range, but there's multiple other band(at least 3 bands in other size), and...
25 July 2023 5,349 5 View
Hi! I am trying to run a model 4 mediation with the xmint option (what was once model 74) but PROCESS produces the following error: "A reference value of X is required for this model." I've...
24 May 2023 6,965 2 View
Hello, I would some help with seeding/plating cells. I counted cells using the hemocytometer. I got 1,025,000 cells per mL. I need to seed 250 cells per well for 36 wells and each well has...
26 March 2023 2,481 3 View
what is the recomended concentration and duration to permeabilize mitochondrial membrane for ICC?
26 March 2023 1,958 9 View
I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...
01 March 2023 749 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View