Dear Doraid Sa ,

I'm sorry but you are wrong. You would just need the 10,000 cells and divide that by your actual cell concentration.

10,000/1,025,000=0,0097560 ml or 9.7 µl

You would then take these 9.7 µl and add that to you 80 ml (with such a small volume you do not need to use 79.9903 ml) and after resuspension you can plate them 2 ml per well.

But you should also expect a high mean variation in the cell number per well when you would count them later.

Best wishes

Soenke

Hello Doraid Sa

You would not need any help since you are right in your calculation.

You have used the correct formula: C1V1=C2V2.

So you add 780ul of your original cell suspension to 79.22mL of culture media, gently pipette up and down a few times for thorough mixing and then add 2ml of cell suspension in each well.

Best.

Dear Malcolm Nobre and Doraid Sa ,

I (still) disagree with your calculation. When you are taking 780 µl of your original cell suspension you will have 799,500 cell in total. When you are now dividing them into 40 well you will end with 19,987.5 cells per well, but you wrote that the goal is to have 250 cell per well. You just need to suspend your 10,000 cells into any designated volume and divide it into the 40 wells.