22 February 2024 2 7K Report

I am hoping that someone can help me find an antibody for GABA, GAD 65 or GAD 67 that works well in marmoset cerebral cortex

Thanks

More Kke Sa's questions See All
How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to write a paper method for interview in a qualitative research ?

Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...

27 April 2024 4,710 1 View

Where to Find Dataset of Requirements Gathering Scripts and User Stories Extracted from Requirements?

Hello, I am looking for a dataset of requirements gathering scripts and user stories extracted from those requirements. Anything similar would be helpful. Thank you.

01 February 2024 4,604 0 View

Please fill my research questionnaire ?

Hello , I hope this message finds you well. I'm a final-year civil engineering student working on a dissertation about the sustainability of ancient Egyptian construction techniques and their...

14 November 2023 987 1 View

How can I confirm the western blot band is identical with my target protein?

I used some polyclonal western blot antibody, and that datasheet size is 45-50kDa I also get the band in expected size range, but there's multiple other band(at least 3 bands in other size), and...

25 July 2023 5,349 5 View

[PROCESS Macro for SPSS] What are the Reference and Counterfactual Values of X?

Hi! I am trying to run a model 4 mediation with the xmint option (what was once model 74) but PROCESS produces the following error: "A reference value of X is required for this model." I've...

24 May 2023 6,965 2 View

Can I get some cell seeding/plating help?

Hello, I would some help with seeding/plating cells. I counted cells using the hemocytometer. I got 1,025,000 cells per mL. I need to seed 250 cells per well for 36 wells and each well has...

26 March 2023 2,481 3 View

Tritox-100 permeabilzation for immunocytochemistry?

what is the recomended concentration and duration to permeabilize mitochondrial membrane for ICC?

26 March 2023 1,958 9 View

Will a signal peptide still be cleaved if GFP is fused in front of it?

I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...

01 March 2023 749 0 View

Wash buffer used prior to cell cytospin?

What is the best buffer for washing blood cells prior to cytospin and fixation? I am not sure whether I use PBS or HBSS?

24 February 2023 2,590 0 View

Similar questions and discussions
Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close