Volume of buffer per each pituitary gland

Morteta H AL-Medhtiy @Morteta-Al-Medhtiy

01 January 1970 0 2K Report

Article Preparation of Mouse Pituitary Immunogen for the Induction o...

Please, can you advice me about the volume of buffer per pituitary gland?

I have only 5 mouse per group and i'm looking to prepare lysate from each one. How much volume of RIPA buffer i need to add for each one? Is it 50 uL buffer ok? Please your experience advice?

Regards

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