I need a free and easy-to-use software to perform volcano plot analysis with my RT-qPCR results. What is your recommendation?
You can use any spreadsheet software (e.g. Excel, Calc, ...) to draw volcano plots.
You can use R
this is an example: http://jura.wi.mit.edu/bio/education/bioinfo2007/arrays/array_exercises_3R.html
R script for volcano plot : http://www.srmcourse.ch/tutorials/Tutorial-8_MSstats.R
other helpful links:
http://artax.karlin.mff.cuni.cz/r-help/library/a4Base/html/volcanoPlot-methods.html
http://artax.karlin.mff.cuni.cz/r-help/library/a4Base/html/volcanoPlot.html
http://www.bio.ifi.lmu.de/files/Software/stabperf/api/doVolcanoplot.html
Easy Volcano plot for gene expression data in Python https://reneshbedre.github.io/blog/volcano.html
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