What are the advantages and disadvantages of using a volatile or a non-volatile buffer for anion exchange chromatography in an FPLC? Is it only related to further processing , like freeze drying or LCMS?
Omar Gonzalez-Ortega
Yes, as long as the buffer is selected appropriately to actually buffer.
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Dominique Liger
Hi there,
You can get rid of it just by evaporation without any usual desalting step. Volatile buffer doesn't basically exhibit any inconvenience.
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