What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Why would one go up and then down with gradient during HPLC?

I think I've seen it previously, but the current example is from this publication https://www.mdpi.com/1420-3049/14/5/1825 where they used the following gradient on HPLC: min ... % MeOH 0 ... 2 20...

09 February 2021 5,422 3 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!

14 January 2021 385 2 View

What fluorescent dye can be used in fixed cells for membrane imaging?

07 January 2021 4,802 2 View

R package for multivariate dispersion as a measure of beta diversity?

Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...

03 January 2021 9,293 1 View

How can I convert the radiance to reflectance or temperature information in MSG2 images with matlab ?

I have meteosat images in the IR and HRV channel I want to transform the radiance into briance temperature on IR images (3x3 km2 resolution) and reflectance in HRV images (1x1 km2 resolution)...

30 October 2020 3,871 1 View

What are some recommended portable devices to measure HRV using ECG or Photoplethysmography (PPG) that I can use it to compare with new devices?

Hello, I am looking for a good (affordable with scientific evidence) device to measure heart rate variability (HRV) by Electrocardiograph (ECG) or Photoplethysmography (PPG), and I will use it to...

27 October 2020 398 5 View

Kubios minimum interval settings and manual artefact deletion for HR (not HRV)?

I am processing data in Kubios HRV Standard and I am running into two technical issues. I am specifically analyzing HR and changes in nonmotor HR, and I'm finding that most of the information...

20 September 2020 1,651 1 View

What is the most proper, cheap and reliable device(portable) which measure HRV for scientific research? Are Polar watchs or other arm bands reliable?

Hello. I want to measure HRV before activity, during activity and after activity. Each measurement will take 5 minutes. I have limited budget and i m searching for a portable device. And it must...

07 March 2020 4,807 3 View

Which HRV frequency range should I use ?

I am trying the determine how effective HRV is at quantifying transient psychophysiological stress in comparison to blood pressure, the experiment comprises of 4 (45 second long) stressors with 3...

01 February 2020 6,546 1 View

Which HRV frequency range should I use ?

I am trying the determine how effective HRV is at quantifying transient psychophysiological stress in comparison to blood pressure, the experiment comprises of 4 (45 second long) stressors with 3...

31 January 2020 3,021 2 View

How to import ECG data recorded with BrainVison Recorder (.eeg files) into Kubios?

For HRV analysis I would like to use Kubios Standard. I recorded ECG with BrainVision Recorder and get .eeg files which cannot be importet into Kubios directly, as the Standard version only...

12 December 2019 6,470 2 View

How to measure HRV-PRV agreement using Bland-Altman plots from Excel (step-by-step guide)?

Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...

25 November 2019 3,736 3 View

Literature Review of Methods of Measuring (Acute) Stress?

Could anyone recommend me a good source describing different variants of measuring acute stress in participants? For example, what are pros and cons of using HRV/cortisol levels or how to combine...

24 November 2019 2,676 1 View

Do HRV variables measure sympathetic and parasympathetic regulation?

Hi, I have a dataset where we have HRV measures during a stress induction procedure. We collected HRV at baseline, during a stress induction procedure and post stress. We collected HRV to...

10 November 2019 2,702 4 View