Vitamines A and E in juice by HPLC ?

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Jerry Decker

Simulated moving bed is a well known industrial chromatography that produces large amounts of food ingredients and related materials.

Mohamed Morsy

I hope to visit the link below

https://www.nacalai.co.jp/global/cosmosil/pdf/Vitamine_Analysis.pdf

Bruce Neagle

Just ultrafilter (0.22 um) and shoot.

Mushtaq Ahmad

Article Chromatographic Determination of Vitamins in Foods

Narong Chamkasem

After you filter, please confirm that your filter does not absorb vitamin A which is very hyrophobic. The filter will behave like the C18 SPE in the 100% aquous and may be absorb your analytes. I am working with vitamin A and related compounds. Even at 80% ethanol 20% water, filter can absorb the analyte. Nyylon is the worst, PVDF is the least. My recommendation is using centrifuge, if possible to eliminat the binding at the filter media.