supercritical carbon dioxide is used in industry for the quantitative extraction of caffeine (e.g. http://www.chemeurope.com/en/products/127801/super-critical-co2-extraction-system.html)
Dear Sir. Concerning your issue about a simple method for caffeine extraction and quantification in solid coffee by HPLC . Caffeine (1,3,5-trimethylxanthine), a mild addicting drug was isolated, purified and characterized from tea (black and green) and coffee. Isolation was done by liquid-liquid extraction using chloroform as an extracting solvent. Extraction was carried out in four steps such as leaching, dye removal, liquid extraction and recrystallization. Crude caffeine was purified by solid phase extraction (SPE) method. The solvent used for recrystallization were toluene and petroleum ether. The purity of caffeine was ascertained by the determination of melting temperature. Pure caffeine obtained from
different samples was characterized by UV-Visible spectrophotometer, TLC, FT-IR and HPLC. In HPLC, 50mM KH2PO4 (pH=2), acetonitrile and methanol (40:8:2) was used as solvent as well as mobile phase. Amount of caffeine in various soft drinks (Cola) commercially available in Bangladesh was also determined by HPLC method. I think the following below links and the attached file may help you in your analysis:
I'm not expert on your question. Because these methods are laboratory and you should ask friends who are specialist in this field. I have been working on complementary scents and therapies. My stuff was not my responsibility.
I'm not expert on your question. Because these methods are laboratory and you should ask friends who are specialist in this field. I have been working on complementary scents and therapies. My stuff was not my responsibility.