4 Questions 1 Answers 0 Followers
Questions related from Krista Mf
Hello, I performed RNA Extraction from ~90 blood samples by TRIzol, got a good RNA and converted it to cDNA, then diluted all the cDNA to 500ng/ul for RT-PCR. I performed GAPDH first, the ct...
21 June 2023 9,574 3 View
Hello colleagues! I performed RNA extraction for my cells using TRIzol, and when I measured the yield using nanodrop, I got two peaks of RNA. I read that it is because of phenol contamination....
17 April 2022 9,308 3 View
Hello everyone, I was running my 10% gel, I ran it on 90V for 30 minutes followed by 120V for around 1:20, then used semi-dry transfer to nitrocellulose membrane. When I developed my blot, I got...
27 February 2022 5,408 0 View
Hello, I would like to visualize the extracellular matrix of breast cancer cell lines MCF-7 and MDA-MB-231 In our facility we have Confocal microscopy. Anyone has any idea of how to perform that?...
14 February 2022 302 0 View
