How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Transmembrane Protein 3-D Coordinate File Preparation Question?

Hello I hope you are all doing well.:) Several transmembrane Pannexin1 3-D coordinate files have been submitted to the PDB, however, I believe all are missing coordinates in some of the Pannexin1...

11 August 2020 4,875 3 View

What are the best metrics for evaluating the quality of non-spherical clusters?

Hi. I'm dealing with clustering of data were the resulting clusters are, in general, non-spherical. Some of them are not convex. What are the best internal metrics for evaluating these kind of...

27 March 2020 5,076 2 View

How can I separate hematite from bornite in a powder?

17 March 2020 570 3 View

How can I access the total number of integration points present in my model using a vumat in Abaqus ?

Hello, I'm using a vumat in Abaqus and i'm storing data associated to each Integration Point present in my model. For the time being, I scale my array to a given large size, say M(10000). I...

08 January 2020 9,622 3 View

I have tried to clone dcas9 into pET22b vector for the past 1month now. And I do'nt always have any colonies. Anyone cloned dcas9 into pET vectors?

I always have pure insert and vector DNA in the region of 1.7-2.0. I use restriction enzymes sall HF and xhol and perform ligation overnight for 16hrs and then do transformation in 10 beta...

30 September 2019 7,178 2 View

Please, has anyone worked on sgRNA labelling? I am finding it difficult to get protocols to label with alexa dye and Qds.?

How can I label single guided RNA (sgRNA) with Alexa 488 dye and quantum dots (QDs)? I will appreciate any paper recommended or detailed protocols.

30 September 2019 6,873 1 View

How do I stop TEM samples being destroyed under electron beam?

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at...

19 August 2019 2,339 1 View

Correct statistical analysis for intervention study pre and post test?

I have ran a study comparing the Daily Mile and HIIT in children to see which is best for improving cardiovascular endurance (Beep test) and anaerobic power (20m sprint). Scores on both test...

29 April 2019 7,457 2 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Can anyone suggest papers in the hybrid auto-mata area that models cyber-physical systems in power systems?

Most of the hybrid automata papers target formal verification of the systems. I am looking for papers that are solving optimal response problems that propose optimal control in power systems under...

07 February 2021 5,233 1 View

What is the reason for the difference in the slope of the hysteresis curve in loading and unloading?

Hello I want to verify a hysteresis curve caused by laboratory results by Abaqus software. The slope of this curve varies in loading and unloading. While the simulation results have the same...

28 November 2020 5,446 9 View

Can I write a research on proposed curriculum design without implementing it?

Good day to you all I am gathering some research-based data supporting a specific way of developing a sample training course in translation. My question: Is it possible for me to just propose the...

09 September 2020 9,265 11 View

Is this a new scam or something reliable?

Dear colleagues, Today I received an email from "[email protected]" with the following text: Dear Author, Congratulations, Your recent publication has been provisionally selected for...

03 August 2020 9,239 95 View

Why fluorescent tag lost after cell harvest?

I used pet16b vector to clone my gene of interest to further purify the protein .I added the fluorescent tag at the c-terminal for further microscopy. After cloning ,samples were sent to sequence...

15 July 2020 3,919 2 View

How can we verify deep neural networks?

How can we verify deep neural networks in terms of reachability, adversarial sample, etc. or from a statistical insight?

25 May 2020 3,577 8 View

How may the real data be Obtained for verification of Black Scholes equation for two stocks model?

It is required in additional information in the submission of a paper in the journal.

20 May 2020 4,991 1 View

Can i perform Verification and validation checks on datasets like AndroPRAguard, Drebin(contain malware and benign mobile apps)?

Can i perform Verification and validation checks on datasets like AndroPRAguard, Drebin(contain malware and benign mobile apps)? Verification and validation checks for data: Verification of...

31 March 2020 8,600 1 View

How to calculate LOD & LOQ in the validation of analytical method using HPLC?

I have injected 5 samples of lowest possible concentrations. I have taken the std deviation of area and slope of the equation obtained for the regression line of calibration to calculate LOD &...

19 January 2020 4,730 7 View