Does anyone know a protocol for gene overexpression in a bacterial strain?

I have a gene cloned in a CMV mammalian vector, transfected into bacterial strain DH5 alpha. I am unable to induce the expression of the plasmid even on adding IPTG (varying concentration) and...

31 December 2012 3,732 8 View

What are the experimental modes of determining the interaction of a protein and a ligand?

What are the experimental modes of determining the interaction of a protein and a ligand (small molecule)?

10 November 2012 472 11 View

Can XRD or any other biophysical methods other than ITC be used to study the interaction between a protein and a small ligand molecule ?

Can XRD or any other biophysical methods other than ITC be used to study the interaction between a protein and a small ligand molecule ?

10 November 2012 5,826 5 View

Is it possible to determine a protein as a cancer biomarker using only cell lines?

Is it possible to determine a protein as cancer biomarker using only cell lines? If yes, what are the methods should be employed to know that the protein is a biomarker?

06 July 2012 7,515 19 View

I have a novel bacterial protein, which while isolating, doesn't come out in the supernatent as it forms inclusion bodies, thereby making it impossibl...

I have a novel bacterial protein, which while isolating, doesn't come out in the supernatent as it forms inclusion bodies, thereby making it impossible to crystallize. Can anyone suggest what I...

04 May 2012 6,497 6 View

Anti- cancer drugs

What can be the main reason for an anti-cancer drug being effective in some individuals and bein ineffective in other individuals???

03 April 2011 8,819 40 View

Cancer cell property

Why does cancer cells tend to become drug resistance??

31 December 2010 4,138 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View