Yogendra Rathore

5 Questions 27 Answers 0 Followers

Questions related from Yogendra Rathore

How to normalize data considering mean and standard deviation?

I am trying to normalize test data with its control. I have 3 independent values of "test data" and 3 independent values of "control data". I calculated mean and standard deviation for both...

04 April 2014 588 6 View

How can I construct a vector to express a human protein for commercial use?

I want to express a human protein in bacterial expression system and eventually I want to commercialize this protein. I am not interested in commercializing the vector itself. Can anybody suggest...

05 May 2013 6,361 8 View

How to make Phenol/CHCl3/Isoamyl Alcohol (25:24:1, v/v) solution using all these three components individually?

Considering the hazardous nature of phenol, I don't want to take any risk of half knowledge. If anybody here has made the Phenol/CHCl3/Isoamyl Alcohol (25:24:1, v/v) solution by himself, please...

12 December 2012 9,486 14 View

Does anyone have any suggestions on how to optimize my PCR experiment?

For the first time, I am facing a strange problem with a purified PCR product. It is around 1.3 kb and I am extracting it from gel. After extraction, when I reanalyze the purified PCR product, it...

12 December 2012 5,059 16 View

How to place a long linker between two domains of the protein? How can I optimize my PCR reaction do achieve it?

I am trying to add a GS3 linker (GGGGSGGGGSGGGGS) between two domains of a protein. For this i have prepared the primers as following: PCR-1 GS3_REVERSE ACT GCC GCC GCC ACC GCT GCC GCC ACC GCC...

12 December 2012 3,858 7 View