We observed two additional bands between 72 and 55 kd in a specific cell type, whereas in HeLa cells on the same blot these were absent.
Alternate RNA splicing leading to larger versions of the protein.
you still see those other bands in your HeLa sample, just lower amounts.
this may be too elementary, but have you tried different anti-Vim Abs? I used to get a nice single band using BM's V9 clone.
So far we could not confirm the ATOH1 protein expression deducted from mRNA expression.
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