17 October 2016 5 831 Report

Hi, I´m a new researcher and I am trying to verify a particular cell line before I start experiments. I am looking at expression of certain key proteins specific to this cell line.

I have been having trouble detecting all these proteins by western blot- getting either faint bands or no bands at all- partly because of my proficiency. 

I am quite keen to know if immunofluorescence staining of these 3 key proteins would be sufficient to achieve this similar goal? that is, if there is immunostaining seen, will that be enough?

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