Hi, I´m a new researcher and I am trying to verify a particular cell line before I start experiments. I am looking at expression of certain key proteins specific to this cell line.
I have been having trouble detecting all these proteins by western blot- getting either faint bands or no bands at all- partly because of my proficiency.
I am quite keen to know if immunofluorescence staining of these 3 key proteins would be sufficient to achieve this similar goal? that is, if there is immunostaining seen, will that be enough?