Verified protocols for differentiating hIPSCs into vascular smooth muscle cell lineage?

Shubhang Bhalla @Shubhang-Bhalla-2

12 June 2024 0 7K Report

Hey all! Thank you for taking the time to read and help if possible!

For some organotypic culture experiments I need to differentiate IPSC into vascular smooth muscle cells

Does anybody have a verified working protocol that I could possibly use?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Shubhang Bhalla's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View

Why does my Modified Gomori Trichrome (LG) Stain muscle fibers red, and how can I improve it?

I am trying to visualize Nemaline Rods in Skeletal Muscle fiber cross sections. I have read about 10 different gomori protocols, and the protocol seems very straightforward. Every paper I read on...

27 June 2024 5,423 2 View

How to predict the efficiency of a very low speed Railgun Actuator/Lorentz Force Actuator?

I'm not an electrical engineer or anything related to that subject, so my apologies if I understood something incorrectly. Well, I read somewhere that the slower the railgun is, the higher its...

23 June 2024 1,082 0 View

Lipid composition of the aortic membrane cells (smooth muscles)?

Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane

19 June 2024 221 0 View

Hello everyone, how could I reduce the background observed in my IFI for NMJ in the lumbrical muscle of mice?

When I stained the presynaptic sites of NMJs in the mouse lumbrical muscle, I observed significant background in my IFI. I fixed them with 0.5% formaldehyde overnight at room temperature....

16 June 2024 3,428 0 View

How is skeletal and respiratory muscle ultrasound being used in the clinical setting, and what are the barriers and facilitators?

Are you a qualified health professional working in the acute adult setting? If yes, then we want to hear from you! Link to survey: https://q.surveys.unimelb.edu.au/jfe/form/SV_aWORJRowoBvxC1U We...

15 June 2024 7,978 1 View