Verification of ChIPseq data?

More Pavla Pavlik's questions See All

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I searched DNA motif in whole genome using FIMO and now I would like to add gene names into the table with founded regions containing my DNA motif. Can anyone help?

10 February 2023 9,069 1 View

How do you find suitable journal or special issues?

Dear colleagues, How do you look for a suitable journal for your manuscript? How do you look for special issues? You contact the editors in advance because it takes many hours to prepare...

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What software do you use for qualitative content analysis of articles (for free)?

Do you use for your qualitative analysis software Diction, QSR...?

13 April 2021 8,128 4 View

How to synthesize pure Bronsted acid sites zeolite?

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Could methanol in formaldehyde influence on crosslink efficiency?

Is it better to use methanol-free formaldehyde for DNA-protein crosslink?

13 November 2017 3,886 4 View

DNA shearing by french press?

Is it possible to shear DNA by french press to get fragment size about 500 bp?

08 October 2017 1,150 12 View

ChIP-seq with DNase?

Hi, could you give me an advice about chromatin immunoprecipitation in bacteria? Is it possible to use DNase for removal nonbinding part of DNA? I mean this: protein will be bind on part of...

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Prediction tool for DNA-binding site?

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What experiences with Primocin do you have?

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How to learn more about SPSS and its Application?

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Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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Kevin Rome

Hi,

You can perform EMSA experiment in order verify the bindding of your regulator of interest on a specific region and also transcriptional fusion to highlight the influence of your protein on the expression the locus close by.

Anukana Bhattacharjee

I agree with Kevin. EMSA and DAPA can confirm protein-DNA binding. As a follow up, you can meddle around the loci (CRISPR or shRNA) to verify if the particular phenotype is perturbed.

Pavla Pavlik

Thank you very much!