Anukana Bhattacharjee

4 Questions 30 Answers 0 Followers

Questions related from Anukana Bhattacharjee

How to monitor half life of peptides?

I am going to test half life of 30 synthetic peptides and was wondering if anyone has a set protocol to test it. Thanks.

29 January 2019 6,239 3 View

Why am I not seeing a sharp peak in smFRET?

I am trying to see a G4 in an smFRET experiment. Although I am using 150mM NaCl and getting a FRET peak at 0.8 but the peak is diffused through 0 to 1 and not a sharp peak. Any one has any idea...

15 December 2016 2,581 7 View

Has anyont faced a problem with making/stabilization of G4 oligo for smFRET?

I am trying to make a G4 oligo with a partial double strand with a biotinylated oligo for smFRET. I started losing G4 FRET in 2 days each time I am making the oligo although I am storing it at 4C...

08 December 2016 6,382 2 View

Any suggestions on why the protein no longer binds to DNA?

I am purifying a single strand DNA binding protein complex. It was binding to radiolabelled oligos and was giving shifts in agarose and acrylamide gel. Suddenly now it stopped binding. I am...

25 February 2015 4,770 6 View