IC50 value from this graph?

01 January 2021 6,848 4 View

What is the best Free/Open Source software for doing Taguchi and other design of experiments and associated meaningful analysis like ANOVA?

Also what are the different efficient DOE and Analysis methods w.r.to machining operation with a specially sintered tool?

03 August 2020 4,553 3 View

What is the current research scenario related to solid lubrication & solid lubricant based cutting tools?

What according to you should be researched/accessed with respect to WC based solid lubricant tools? What is your view with respect to future research in this area? (For e.g. Tribological study...

19 July 2020 3,232 6 View

Can I freely use figures from open access journals/articles by citing the article?

I am writing a review paper. In my paper, can I use figures from "open access" journals/articles etc provided I cite them properly? (i.e. without have to go for requesting permission for using)....

02 July 2020 9,363 15 View

How many references to cite for a review article?

I am currently working on a review article. however, I am not able to get more research papers for it. Still my article is quite having content as the data is very rare. Is there any minimum...

17 May 2020 3,870 20 View

How does one mine data from the Cancer Therapeutics Response Portal?

I am interested in using the Broad's Cancer Therapeutics Response Portal to see the correlation between the expression of a particular gene of interest and resistance to a few specific drugs. I...

23 February 2020 112 3 View

How to change the pre-trained Caffe model to accept one channel input(Gray Scale Image) instead of an RGB image?

I apply holistically nested edge detection method for Edge and Boundary Detection in Images. The model can accept only the RGB image. Can anyone help me to accept one channel input image(Gray...

27 January 2020 5,512 3 View

Under what circumstances can we consider mass equivalent to weight and vice versa ?

Can mass be always considered equivalent to weight ?

17 September 2019 7,790 3 View

How to do mechanism study in photo-catalytic degradation of pollutant?

Dear Friends, I am currently doing some experiment where i prepared some nanomaterial and now trying to degrade dye molecule under visible and UV light condition. I need help regarding setting up...

21 May 2019 1,567 1 View

What would be right (to be ideal or Potential) concentration (ug/ml) of a plant crude drug extract on MCF-7 cell lines?

What would be right (to be ideal or Potential) concentration (ug/ml) of a plant crude drug extract at 50% inhibition (IC50) values against MCF-7 human breast cancer cell lines? I have red an...

14 May 2019 4,543 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View