Dear all,
Hello, I do not have any experience on ELISA experiments.
Thus, I have a problem whether I can exchange VEGF121 and VEGF-A into rhVEGF121 because VEGF-A Human ELISA kit from ThermoFisher Scientific is too expensive. Thus, I am wondering if I can use VEGF DuoSet from R&D System instead of applying Thermo kit.
The 'rh' means human recombinant.
100
VEFG-A Human ELISA kit from ThermoFisher Scientific detects human VEGF-A, and I know that the VEGF-A gene has isoforms, which are VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206.
The VEGF DuoSet from R&D System recognizes rhVEGF165, rhVEGF121, and rhVEGF165b.
Can I use VEGF DuoSet not using VEGF-A Human ELISA kit?
I have attached the two types of ELISA kit protocols.
Please answer my question. Thank you so much.
The Human VEGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. ... Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody.
https://www.thermofisher.com › elisa
VEGF Human ELISA Kit - Invitrogen - Thermo Fisher Scientific
An ELISA kit is a commercially available ELISA test that usually contains pre-coated polystyrene plates, detection antibodies, and usually all of the chemicals needed to perform an ELISA test.
https://www.medicinenet.com › article
ELISA Test Steps, Results, Types & Procedure Risks - MedicineNet
Vascular endothelial growth factor (VEGF) is considered the master regulator of angiogenesis during growth and development, as well as in disease states such as cancer, diabetes, and macular degeneration.
https://pubmed.ncbi.nlm.nih.gov › ...
The function of vascular endothelial growth factor - PubMed
Typically, VEGF in either serum or plasma samples from anti-VEGF–treated patients is measured with a sandwich enzyme-linked immunosorbent assay, with the R&D Systems kit (Minneapolis, MN, USA) being the most commonly used.
https://iovs.arvojournals.org › article
Comments on Assays Used to Measure VEGF in the Presence of
https://www.rndsystems.com/products/human-vegf-quantikine-elisa-kit_dve00,kindly check this link.