Hi Riham

You are able to measure the protein content of your sample in Laemmli buffer using the nanodrop. Set the nanodrop to read protein concentration and load 1uL of your sample. I would include a known BSA standard as a control to double check that you will get a correct measurement.

Cheers,

Pieter

I did use the BSA protocol on the nanodrop with my standards, however, the wavelength of the buffer is interfering with the measurements!

Glycerol absorbs strongly in the UV. You can try making your own Lamelli buffer using filtering the glycerol through activated charcoal , which should decrease the absorbance. The lamelli buffer needs to be used as a blank. If the Lamelli buffer already contains Comassie Blue or another dye you will not be able to detect the protein.

If you absolutely need the Lamelli buffer to elute, you can use other methods like BCA analysis or fluorescamine derivatization.