the takara website suggests the following for human genomic dna amplification

Component VolumeFinal concentration

Human genomic DNA (500 ng/µl)1 µl500 ng per 50 µl reaction

10x LA PCR Buffer II (Mg2+ Plus)5 µl

1XdNTPs 8 µl 400 µM each

Sense Primer (20 pmol/µl) 0.5 µl 0.2 µM

Antisense Primer (20 pmol/µl) 0.5 µl 0.2 µM

Takara LA Taq0.5 µl 2.5 U per 50 µl reaction

Sterile distilled water 34.5 µl