There is a big confusion regarding the reaction mixture for Long-Range PCR?
Paul Rutland
the takara website suggests the following for human genomic dna amplification
Component VolumeFinal concentration
Human genomic DNA (500 ng/µl)1 µl500 ng per 50 µl reaction
10x LA PCR Buffer II (Mg2+ Plus)5 µl
1XdNTPs 8 µl 400 µM each
Sense Primer (20 pmol/µl) 0.5 µl 0.2 µM
Antisense Primer (20 pmol/µl) 0.5 µl 0.2 µM
Takara LA Taq0.5 µl 2.5 U per 50 µl reaction
Sterile distilled water 34.5 µl
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