Using HEPES as a sample buffer in EMSA?

20 April 2022 0 8K Report

Hi,

I'm running EMSA gels on a protein-DNA mixture (I'm visualizing the DNA) and I've been using HEPES as my sample buffer. My DNA bands are barely migrating even after running at 150V for 70 mins. Do you have any suggestions as to how to get my bands to migrate further and why this is happening?

My gel is 10% acrylamide made with and run in TBE buffer/

Thanks!

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