How to interpret multilevel model with categorical variables (fitted using brm() in R)?

I am using brm() in R to fit a multilevel model for repeated measures data. The two predictors are both categorical. One is `Task` with two levels (`LO` and `ID`), another one is `Condition` with...

10 November 2020 712 4 View

Can Scheirer Ray Hare test be used in unbalanced data and sample size <5?

Hi everyone, I have a "tricky" dataset with two factors but is unbalanced and sample size small than 5. And this data is not a normal distribution but a bimodal. So I think ANOVA is not very...

29 July 2020 2,707 1 View

Has anyone ever done research on employment for people with disabilities especially in public service?

I am currently doing my research on employment rate of people with disability in public service. I would be glad if someone has done or is doing the same topic.

19 March 2020 4,631 3 View

How to statistically analyze bad replication experiment data ?

11 March 2020 7,545 7 View

What is wrong with the plates?

I conducted a plaque assay in a 24-well plate. All 12 of the plates had the same problem shown in the attached pictures. I did a mock assay before this and it went well without any problems. Does...

25 December 2019 3,833 2 View

Ways to avoid dystocia of transgenic mice?

I am trying to breed Rag-/- mice and I found high percentage of female dead by dystocia. Is there any way to avoid this issue?

10 September 2019 3,668 1 View

Reliable conjugated and unconjugated IL17-F anti-human antibody for flow cytometry?

Dear all, can someone kindly recommend a reliable fluorochrome-conjugated IL17-F anti-human antibody and also an unconjugated one please? I am studying CD3+ Lymphocytes from healthy volunteer...

19 February 2019 3,843 3 View

How to measuring extracellular ATP level?

How to measuring extracellular ATP level in tumor specimens, not the culture cell? Is there are commecial Kits available?

17 November 2018 1,053 1 View

How to analyze flow cytometry data?

I analyzed the data of my flow cytometry assay. Data of Capture-1 is about cell apoptosis induced by nanoparticles in cell culture. 1. Was the gate set correctly? 2. Why did the clusters of the...

24 April 2018 1,916 2 View

Is it possible to run SDS-PAGE with sample of Nanoparticle combined with Albumin?

I add nanoparticle and Albumin together, incubator 24 hours. I run SDS gradient gel with this combination after 10min, 70 degree denature. I got something like half way waterfall on the gel....

09 January 2018 4,804 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View