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Questions related from Kay Ab.
Dear all, I wonder if it is convenient to use BL21(DE3)PlysS without adding glucose to express protein of interest? if its okay, do you have a reference ?
06 May 2024 3,419 0 View
Hi everyone, In my readings about C. diff. ribotypes, I found a ribotype the researcher refers to it as RT 014/020. I wonder if it refer to 014 and 020 strains or 014 to 020 strains or what...
13 November 2023 3,240 0 View
Hello everybody, I purified my protein and when I run it on SDS-PAGE, a small molecular weight sized band (10 kDa) appears. Do you have any idea what that could be? an image of the SDS-PAGE is...
10 July 2023 5,309 3 View
Dear all, I cultured C. difficile into BHI under anaerobic condition and when the cells reached the mid log phase, I centrifuged them and washed them twice with pre-reduced PBS (pH 7.4). Cells...
23 August 2021 4,968 0 View
