Using a 10 mg of extract, calculate the stock solution of 1 mg/ml which later needs to be diluted into various concentration 100, 50, 10, 5, 1 and 0?

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Alexandra Johnson

You would need 10 mL of solvent for 10 mg of extract to make 1 mg/mL. The dilutions can't be calculated without knowing the units for the concentrations.

Nr Izzatie

thankyou Alexandra Johnson . but for question 2 how to calculate the concentration? is it use M1V1=M2V2 ?

Nr Izzatie Yes, that is the equation you would use. Just remember to keep the units the same on both sides - ie. 1 mg/mL = 1000 µg/mL if you're calculating for a µg/mL concentration.

thankyou so much Alexandra Johnson