Hi,

I have a question regarding the use of Human TruStain FcX™ (Fc Receptor Blocking Solution) when doing flow cytometry staining for FcƐRI on dendritic cells. Before starting to use the above-mentioned blocking solution, I used human serum as a blocking agent during the staining procedure but I noticed a lot of background (and bad isotype control). When using the Human TruStain FcX™ (Fc Receptor Blocking Solution), the level of FcƐRI measured is lower and the plots look better (isotype control good as well). Could this change be due to binding of the blocking agent to my receptor of interest? Can it interfere with my specific flow cytometry antibody instead of blocking only a-specific bindings? Thank you in advance.