Can I use anti-bovine Ab as a secondary Ab (conjugated Ab) in the ELISA test for sheep serum? or it should be anti-ovine Ab only?
Dear Khaleel Z. K. Al-Alo
Check these if helpful
https://www.jacksonimmuno.com/secondary-antibody-resource/technical-tips/controls-diluents-blocking/
https://www.thermofisher.com/eg/en/home/life-science/antibodies/secondary-antibodies/secondary-antibodies-target-species/anti-mouse-secondary-antibodies.html
I think it's difficult to speculate without actually testing it. Can't you test whether it works with small number of samples?
Alternatively, if your anti-bovine antibody were obtained from a commercial source, you can check with the manufacturer. However, even if they say that it should work, you have to do the testing yourself. Thanks.
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