A case of progressive large fiber peripheral neuropathy with sparing of any CNS effects causing loss of motor function and sensory reception. What are treatment options/
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.
26 January 2021 8,733 2 View
Hello everyone, I am a researcher working on a project aiming to develop an assay for Haemoglobin quantification. In our assay, prior to HGB quantification we dilute the sample in a dilution...
20 November 2020 7,316 2 View
Hello everyone I am running a thermo-mechanical simulation on a Linux Cluster. In my Project view all the component systems seem to have solved. There is a file.rst file and and solver.out...
14 October 2020 2,631 3 View
The pitch value set in music and guitar group in tablature are connected by adjunction of tangent-cotangent bundles. Tuning g is the tangent gradient to the flow of pitch on guitar. It determines...
04 April 2020 2,681 15 View
I want to write a script that asks the user to enter the PDB file so that the script can be entered (it prompts the user to enter it), the user enters the 4 character code, then the rest of the...
23 February 2020 1,184 1 View
I just read a new paper posted here by Constantino Manuel Torres. However when I put his name in the search engine here, it says there is now one here with that name? I wanted to send him a...
13 July 2019 2,496 3 View
I recently introduced a Lenti-Puro Guide vector containing a specific sgRNA sequence into a cell line. I would like to sequence the ddCas9-sgRNA cell lines to confirm that each sgRNA is accurate.
04 February 2019 6,681 3 View
Hi, A friend of mine suggested that the reaction that makes Salmonella colonies appear black on XLD or TSI agar might show an increased rate at a slightly lower pH. I was thinking of adding a bit...
24 July 2018 2,916 7 View
Hello All. Can anyone suggest antibodies against MMP2 and MMP9 that work well for immunohisto in the mouse heart? Thanks in advance. Bruce
24 November 2016 7,284 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...
23 February 2021 5,503 1 View
I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.
22 February 2021 4,323 3 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
Hi sir, 1.can you please suggest to me how to convert the EDF (European Data format) file into a Mat(Matlab) file for EEG datasets?. 2. Also suggest some publicly available EEG Motor Imagery...
15 February 2021 7,620 1 View
Hi RG community, Like the title shows ,i was wondering if choosing the GA for this kind of Optimizaiton is a good choice? For example we have a population of 100 (for 7-8 Optimization Variables),...
14 February 2021 1,160 7 View
Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...
10 February 2021 1,797 5 View
Dear All, I am working on electric motor design in Ansys Maxwell. I have written optimization code in MATLAB. I want to run this code in ANSYS MAXWELL and generate FEA results. Does anyone know...
10 February 2021 1,279 1 View
Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...
09 February 2021 5,334 8 View