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Questions related from Nathaniel Zhu
I'm trying to remove dead cells from human Mononuclear cell culture without either slow centrifugation or ficoll. I would like to remove dead cells by pulvevrising them into debris (smaller than...
16 October 2018 8,431 1 View
Usually I'd put vials of cells mixed with a Freeze mixture containing DMSO into corning foam boxes into the -80 that cools cells down at 1C/min for 2 days before transferring to the Liquid...
05 March 2018 9,975 0 View
I use 1.130g/mL Percoll. Then I make a 46% mixture of Percoll and X-Vivo 20 with 5% Serum. So like 46mL Percoll, and 54mL XVIVO20+5% Serum. And then I layer 20mL of 46% Percoll in a 50mL tube,...
11 November 2017 7,635 1 View
I'm trying to extract the White Blood cells from Blood for cell culture. I'm currently using LSM to get the mononuclear cells but I think it would be easier just to lyse the red cells directly...
26 October 2016 3,770 3 View
I'm doing RNA extraction with TRIZOL, but I just ran out. I saw some bottles identical to the TRIZOL so I assumed I had more but it turned out to be "buffer saturated phenol". is this the same as...
05 September 2016 4,478 3 View
I've been researching various antibiotics and their uses from gentamycin (which is used in my cell culture media) to Penicillin and streptomycin. But I'm also wondering, if those cheap off the...
15 April 2016 1,646 1 View
We are trying to detect and quantify the presence of KLF2 which should appear as we have memory cells (CCR7+/CD62L+). We're using western blotting for that and we've tried both the traditional...
17 November 2015 6,309 2 View
