Universal/Bacterial DNA Amplification - Best cycling conditions/protocol for use of 1492R and 1070F primers ?

01 February 2022 1 2K Report

I am looking to use 1492R universal primer and 1070F bacterial primers to verify the presence of PCR amplifiable DNA in my eDNA samples. Does anyone have any recommendations for cycling conditions or a protocol to use to get the most of out of the primers?

Abhijeet Singh

You have to fine tune your PCR protocol anyway with your polymerase and sample type. Better to use gradient PCR to adjust parameters which suits your specifics.

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