Unhappy Sf9 cells?

01 January 1970 1 5K Report

I am currently culturing Sf9 insect cells for a protein expression and noticed some tadpole-like cells (red circled in the picture). They seem to reduce/disappear when cells are close to confluence. Are they just unhappy Sf9 cells, or could it be a contamination?

Thanks

Muhammad

Kerry S Vistisen

They are normal. The clue is that they look like the other cells once close to confluency.

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