I have tried several times to transduce MC38 cells with an empty GFP viral vector to no avail. I've tested the tumor cells and the producer cells (Phoenix-Eco) for mycoplasma and they're both negative and I've had no luck doing a spin infection. I transduced these cells in tandem with CT26 and 4T1 which I was able to transduce so I know there isn't a problem with the vector, reagents, or protocol I'm using so I'm completely stuck. Any help would be greatly appreciated.
Thanks so much Jason! I tried transducing with Phoenix-Ampho cells and it worked great. Thanks again for your help!
Sam
I recently transduced this same cell line (MC38) with my gene of interest via Lentivirus and I got some positive clones. However, the cell growth reduced after transduction and I am thinking what could be going on... Does anyone have any suggestion for me to overcome this?
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