Annette E. Sköld

4 Questions 6 Answers 0 Followers

Questions related from Annette E. Sköld

Shelf life of TRIS?

I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...

04 April 2016 9,849 6 View

Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!

08 August 2013 9,774 2 View

How to measure DC induced polarization of human naive T cells?

Can I look at human naive T cell polarization using DCs and T cells in a allo-setting? Hence, induce T cell activation and proliferation with DCs displaying non-self MHC complexes. Given that the...

01 January 2013 7,840 5 View

Why should one dilute the buffy coat before ficoll separation?

Does diluting the buffy coat in EDTA buffer before ficoll separation result in higher cell number output or cell viability? To load the buffy coat quicker into fewer tubes reduces the processing...

01 January 2013 7,200 22 View