I have obtained two bands from the DNA samples of Glaciozyma sp.

Has anyone works on Glaciozyma encountered the same issue.

the second band (~5000bp) is clear and distinct doesn't look like RNA contamination.

i have repeated the same extraction from a different batch of colonies cultured at different time (liquid culture)

and consistently they all have two bands.

this is a 1% agarose gel with 1kb ladder