I isolated DNA from whole blood using the salt-out method. The Nanodrop results show good quality and concentration, but after running on an agarose gel, no bands are visible. Can anyone suggest a solution?
It may simply be that your dna is very high quality so is very high molecular weight (size) so if your gel percentage is too high then the dna is unable to diffuse in the gel so remains around the wells. Agarose percentage for large dna molecules should be less than 1%
according to your images, it seems that the DNA band is clear, but the gelation quality is low, which could be due to the addition of a coloring agent at high agarose temperatures. And you can also reduce the percentage of agarose .
DNA quality values were 1.0, 1.5, and 1.8 (A260/A280 ratio), and the samples were analyzed using 0.8% agarose gel electrophoresis.
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