I am using 4-20% biorad precast mini gel and protein ladder is also from biorad. I have attached the image of membrane. Running voltage- 100 V for 1 hr at room temperature and 70 V for 2.30 hr at 4C. I am stuck with the crooked ladder. Lower bands of ladder become wider and it happens twice back to back. Please share the suggestion if you had the same experience or know how to correct it.
Thanks