I want to make a fusion protein that forms a stable trimer (like GST dimer). Which fusion protein is good for that purpose?
I am measuring protein-protein interaction using fluorescence polarization. Is there a tool to calculate theoretic fluorescence polarization vs. M.W. using dyes with different fluorescence...
31 December 2016 4,880 2 View
My protein has a DNA binding domain and has a pI of 6. It is overexpressed in E coli and his tagged. After Ni column, I found there is a lot of DNA in my sample based on 280/260. On the SDS-PAGE,...
03 April 2013 5,899 37 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I am working on centrosomal proteins. I overexpressed my POI and then I did SNAP capture pulldown followed by mass spec and I got approximately 700 protein hits. I am trying to validate specific...
03 July 2024 5,701 0 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...
27 June 2024 7,318 3 View