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I am measuring protein-protein interaction using fluorescence polarization. Is there a tool to calculate theoretic fluorescence polarization vs. M.W. using dyes with different fluorescence...
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I want to make a fusion protein that forms a stable trimer (like GST dimer). Which fusion protein is good for that purpose?
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My protein has a DNA binding domain and has a pI of 6. It is overexpressed in E coli and his tagged. After Ni column, I found there is a lot of DNA in my sample based on 280/260. On the SDS-PAGE,...
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